Qualitative reseach on fast plants
The scientific test carried out this semester, involved qualitative research to see the abstraction of inherited traits in the plant; Brassica Rapa. The dummy used by the class to come up the hypothesis in discovering the form of inheritance in the Brassica Rapa plant is known as the Mendelian model. When the experiment was concluded, Facts gathered indicated that the dominant popular purple gene called anthocyanin conformed to Mendel’s laws. The basis for the test was at best trial and error so the outcome was considered inconclusive.
Introduction
The primary concept of hereditary was founded by an Australian scientist by name; Gregor Mendel after he concluded some experiment in his garden. In 1854, Mendel started began carrying out tests touching on the concept of plant hybrids communicating hereditary traits. As such, a phenotype serves as a basis for observing the inheritance patterns in genotypes and explores whether they adhere to the laws of Mendel. After Mendel carried out his experiments with pea plants, he came up with two proposals which are referred to as the Mendelian genetics. The first law states that during the formation of gametes, paired alleles usually separate and this is referred to as the ‘The Law of Segregation.’
The second law, ‘The law of Independent Assortment,’ states that during gamete formation, the alleles separate independently (Pearson, 12).In this case, the plants used to experiment were the fast plants which contained strains of the Brassica Rapa gene. The gene responsible for the purple formation pigment in the Brassica Rapa plant is called anthocyanin (Gould, 6). The information according to the laboratory manual stated that the breeding cycle from seed to seed for the Brassica Rapa plant is thirty-five days. Also, the phenotype refers to the observed characteristics of an individual (Rollo, 4) Because of this, the plants that belong the Brassica Rapa family are instrumental as models in the research and experimentation of genetics and hereditary transfer.
Materials and Methods.
Before starting off the experiment, the following materials were obtained to complete the test successfully and promptly which included; seeds with designated phenotypes, a collecting pan for seeds, some small envelopes, tap for labeling, Styrofoam quads, and fluorescent lighting. Furthermore, we obtained some water, a dropper, fertilizer pellets, a pot for mixing, a watering tray and a petri dish fitted with a filter paper. Table 1 shows the procedure followed.
|
Approximate date |
Date |
Activity |
Initials |
|
Day 1 |
|
F1 hybrid plants planted and water checked. |
|
|
Day 4 or 5 |
|
Seedlings observed and phenotype recorded |
|
|
Days 14, 16, 20 |
|
Pollinate on 3 days; pollinated around 6 to 8 followers |
|
|
Days 20 to 39 |
|
Buds and shoots removed |
|
|
Day 39 |
|
F2 seeds harvesting and germinated in petri dishes. |
|
|
Day 42 |
|
Number of each phenol type recorded |
|
Table 1.
Below is the table for the results of the experiment.
|
Section |
# of seeds planted |
Purple stem plants |
Green stem plants |
% Germinated |
|
1 |
536 |
219 |
127 |
65% |
|
2 |
561 |
190 |
143 |
59% |
|
3 |
701 |
309 |
120 |
61% |
|
4 |
407 |
148 |
127 |
68% |
|
5 |
867 |
400 |
154 |
64% |
|
6 |
614 |
114 |
145 |
42% |
|
7 |
1295 |
334 |
340 |
52% |
|
8 |
536 |
111 |
116 |
42% |
|
9 |
240 |
79 |
55 |
56% |
|
10 |
599 |
127 |
322 |
74% |
|
11 |
185 |
9 |
6 |
8% |
|
12 |
1136 |
445 |
188 |
56% |
|
13 |
248 |
64 |
64 |
52% |
|
14 |
412 |
163 |
106 |
65% |
|
15 |
315 |
154 |
74 |
72% |
|
16 |
1384 |
639 |
323 |
70% |
|
17 |
1262 |
556 |
315 |
70% |
|
18 |
717 |
147 |
229 |
52% |
|
19 |
737 |
413 |
192 |
82% |
|
20 |
866 |
302 |
154 |
53% |
|
21 |
224 |
54 |
26 |
36% |
|
22 |
909 |
429 |
175 |
66% |
|
23 |
1242 |
441 |
382 |
66% |
|
H1 |
194 |
54 |
44 |
50% |
|
H2 |
637 |
158 |
88 |
39% |
|
|
Purple-stemmed plants |
Green-stemmed plants |
|
Observed (o) |
148 |
127 |
|
Expected (e) |
305 |
101 |
|
Deviation (o-e) or d |
-157 |
26 |
|
Deviation2 or d2 |
24,649 |
676 |
|
d2/e |
87.4 |
6.7 |
The Chi square calculation formula is; X2 = total of (observed-expected)2 over all cases
Expected
As such, X2 is 94.1.
Conclusion
To make a conclusion, the data obtained had too many errors since it did not disagree or agree with the hypothesis. However, aggregate data for the class supports the hypothesis that the dominant gene is the purple pigmented plant.
Works Cited
Chyi, Y-S., M. E. Hoenecke, and J. L. Sernyk. "A genetic linkage map of restriction fragment length polymorphism loci for Brassica rapa (syn. campestris)." Genome 35.5 (1992): 746-757.
Morgan, Judith Giles, and M. Eloise Brown Carter. Investigating biology. Benjamin/Cummings, 1996.
Pearson K. 1904. Phenotypes : their epigenetics, ecology and evolution [Internet]. 1st Edition. New York [NY] Chapman and Hall.
Radke, Sharon E., Joann C. Turner, and Daniel Facciotti. "Transformation and regeneration of Brassica rapa using Agrobacterium tumefaciens." Plant Cell Reports 11.10 (1992): 499-505.
Weis, Arthur E., and Tanya M. Kossler. "Genetic variation in flowering time induces phenological assortative mating: quantitative genetic methods applied to Brassica rapa." American Journal of Botany 91.6 (2004): 825-836.
Qian, Wo, et al. "Introgression of genomic components from Chinese Brassica rapa contributes to widening the genetic diversity in rapeseed (B. napus L.), with emphasis on the evolution of Chinese rapeseed." Theoretical and Applied Genetics 113.1 (2006): 49-54.
Academic levels
Skills
Paper formats
Urgency types
Assignment types
Prices that are easy on your wallet
Our experts are ready to do an excellent job starting at $14.99 per page
We at GrabMyEssay.com
work according to the General Data Protection Regulation (GDPR), which means you have the control over your personal data. All payment transactions go through a secure online payment system, thus your Billing information is not stored, saved or available to the Company in any way. Additionally, we guarantee confidentiality and anonymity all throughout your cooperation with our Company.